催し 微生物機能の戦略的活用による生産基盤拠点					京都大学21世紀COE
		講演会・セミナー 　 21世紀COEプログラム「微生物機能の戦略的活用による生産基盤拠点」 特別セミナー 「企業におけるバイオ戦略と研究開発」 平成19年3月7日（水）15:30〜17:00 農学研究科２号館101　応用生命科学専攻第４セミナー室 Enzymes in Organic Synthesis From Commercial Enzyｍes to Tailor-Designed Biocatalysts Prof. Dr. Uwe T. Bornscheuer （ドイツ グライフスバルト大学 生化学研究所・教授） 連絡先：清水　昌（Tel: 6115）
		An impressive number of examples has been developed in the past decades for the application of enzymes in organic synthesis, with hydrolases as the most frequently used biocatalysts . Whereas initially, commercial enzyme preparations have been used 'straight from the bottle', the current trend is to tailor-design the biocatalyst using methods of molecular biology and protein engineering. Examples include the functional expression of a recombinant pig liver esterase (PLE) isoenzyme in the yeast Pichia pastoris, directed evolution to design esterase mutants showing altered substrate specificity as well as substantially increased enantioselectivity and the identification of a sequence motif determining activity towards tertiary alcohols, which could be substantially improved by rational design. In addition the identification of protease substrates by phage display will be shown. Also, the application of Baeyer-Villiger monooxygenase in organic synthesis will be presented. More recently, we turned our interest to catalytic promiscuity. First results will be shown as well.